Introduction to HEPES buffer CAS NO.1185-53-1

1. Factory production, more favorable prices, and regular products are available in stock;

2. Desheng is a manufacturer of a series of biological buffer products, with a wide range of products, which can provide you with more comprehensive services;

3. Desheng has been in this industry for 14 years. Our products are independently developed and manufactured. Biological buffer has high purity and guaranteed product quality.

4. Desheng has been engaged in foreign trade since 2010, and we have our own export right.

HEPES (4-2-hydroxyethyl-1-piperazinyl-ethanesulfonic acid) is an organic substance used to prepare buffer solution. It is widely used in cell-cell culture to maintain a stable physiological pH value. Its PKA at 20 ° C is 7.55. HEPES is used to protect frozen enzyme solutions from freezing induced pH changes. Considering the phototoxicity of HEPES (hydrogen peroxide will be produced if exposed to ambient light), this buffer should be stored in a darker environment after being configured as a solution, so as to maintain its normal function in subsequent experiments.

Biological buffer HEPES powder

HEPES is frequently used in cell-cell culture because it has been proved to be a good maintainer of physiological pH. it has advantages over other buffers (such as carbonate buffer). In addition, it can avoid cell-cell growth changes caused by carbon dioxide release. The pH value of HEPES decreases with the decrease of temperature, but the dissociation constant PK of phosphate and bicarbonate buffer hardly changes with temperature. The behavior of HEPES is similar to that of water. When the temperature decreases, its dissociation constant will decrease. HEPES can be used to maintain the structure and function of enzyme at low temperature.

HEPES produced by Desheng is white crystal powder with high purity and good quality. The main content is ≥ 99.50%, iron ion ≤ 5ppm, sulfate ≤ 0.05%, and heavy metal residue (PB) ≤ 10ppm. A small amount of trial sample powder can be provided and configured into a solution for testing.

As a professional manufacturer of biological buffer, Desheng has 16 years of production and R & D experience since its establishment in 2005. At present, Desheng's biological buffer products include HEPES, mops, Tris, taps, caps, bicine, EPPs, PEP and a series of biological buffer. If necessary, please contact us!