The difference between crude heparin and finished heparin sodium CAS NO.9041-08-1

1. Direct selling by manufacturers, preferential price and spot supply;

2. Desheng is a manufacturer specializing in the production of a full set of vascular additives. There are many kinds of products, which can provide you with more comprehensive services;

3. Desheng has been engaged in blood vessel collection industry for 14 years. Our products are independently developed and produced. We have the ability to solve most of the problems in vascular collection and provide customers with safer services.

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In the eyes of most people, heparin is a drug. In fact, in addition to medicinal purposes, heparin has many uses, such as in vitro diagnosis, biochemical testing, blood collection and storage, or cosmetics. These heparins are purified and processed from crude heparin before they are made into finished heparin sodium.
source of heparin
Heparin is called heparin because it was originally found in the liver and is a natural anticoagulant substance present in many organs of mammals, with the highest content in the lung and intestinal mucosa. Unfractionated heparin is a kind of aminodextran sulfate extracted and refined from pig intestinal mucosa or bovine lung. It is a mixture with a molecular weight range of 3000-30000KD and an average of about 15000KD. Fragments of aminodextran sulfate after unfractionated heparin cracking, namely low molecular weight heparin heparin calcium, enoxaparin, dalteparin, etc., have an average molecular weight range of 3000-8000KD.
Preparation of crude heparin
After the fresh pig intestines (or after the natural thawing of frozen pig intestines) are carefully washed with water, enzymolysis is carried out: the raw materials are fully stirred, and the pH value is finely adjusted to 8-9 with a small amount of dilute lye. Enzymatic hydrolysis at 37-40 degrees for 3-4 hours. Add about 5% of the total weight of the feed liquid (containing NaCL ≥ 95%, calcium magnesium calcium salt < 0.5%) to make it evenly mixed, then heat up to 90 degrees, keep the temperature for 30 minutes, finely filter, and adjust the filtrate to its pH= The ion exchange adsorption treatment is carried out in the range of 9.0-9.5. Elution, suction filtration, combined supernatant and filtrate, finely adjusted PH=6.0-6.5, added 1.5 times the amount of 95% ethanol to precipitate overnight, the next day, carefully dried and sucked out the supernatant, collected the lower sediment, suction filtered to dryness (The mother liquor can be used in the eluent before adjusting the pH value). After vacuum drying, crude heparin is obtained.
Refinement of finished heparin sodium
The crude heparin is completely dissolved in 2% sodium chloride solution to make a solution with a solubility of about 8%. During this process, the temperature can be appropriately raised to help dissolve. The above material was finely adjusted to PH=8.0-8.2 with 5mol/L sodium hydroxide solution, heated to 78-80 degrees, 0.15-0.2mol/L potassium permanganate solution was added for oxidation, finely filtered, and the material liquid was treated with " Filter by "sterilizing filter", add 3-4 times of 95% ethanol for precipitation, dehydrate with acetone, grind to fine, and dry in far-infrared vacuum (50-60 degrees) to obtain heparin sodium fine product.
It can be seen from the above that crude heparin is the raw material, and the finished heparin sodium can be obtained through further refining. Desheng currently provides heparin raw materials including blood collection tube heparin, enoxaparin, cosmetic heparin, etc.