Tris buffers are used for many purposes in electrophoresis CAS NO.77-86-1

1. Factory production, more preferential prices, regular products are available in stock;

2. Desheng company is an enterprise producing biological buffer products. There are many kinds of products, which can provide you with more comprehensive services;

3. Desheng has been in the industry for 16 years. Our products are self-developed and manufactured. Biobuffer has high purity and guaranteed product quality.

4. Desheng began to do foreign trade in 2010. We have our own right to export.

A high-quality Tris buffer is an essential part of electrophoresis. It allows current to pass through the sample while resisting pH changes throughout the solution. The choice of buffer depends on the isoelectric point of the sample being analyzed. In DNA electrophoresis, commonly used buffers are Tris-Acetate-EDTA and Tris-Borate-EDTA. In protein electrophoresis, SDS (sodium dodecyl sulfate) is usually used. These buffers help separate samples into readable gels. Other buffers can help improve results or extend readings at specific molecular weights, depending on the sample.

Recently, I have obtained some knowledge about electrophoresis buffers through professional literature, and I would like to share and discuss with you here.
The role of Tris-acetate-EDTA and Tris-borate-EDTA in agarose gel electrophoresis:
Tris is a strong base and boric acid is an acid, and the combination of the two keeps the pH in the neutral range of 8 to 8.5. Under these alkaline conditions, DNA is protected and can be separated correctly. EDTA plays an important role in this binding. EDTA is a chelating agent. It chelates Mg2+ ions required by DNAse enzymes as cofactors. DNAse is an enzyme that cuts DNA into small fragments. Therefore, by adding EDTA, our DNA is protected from enzymatic activity. In addition, the buffer will neutralize the charge of the water molecules. Under the action of electric current, water molecules are decomposed into H+ and OH-. H+ ions can react with PO3- of DNA. Therefore, the negative charge of the buffer neutralizes the charge in the water and protects the DNA.
Although Tris-Acetate-EDTA and Tris-Borate-EDTA work the same in electrophoresis, they each have their own advantages and disadvantages. Tris-acetate-EDT buffer can interfere with enzymatic reactions and protect DNA, while Tris-borate-EDTA buffer cannot.
In conclusion, the use of Tris-Acetate-EDTA and Tris-Borate-EDTA derived from Tris buffers in electrophoresis is very important, which is why Tris buffers are in short supply in today's market. one. It was very difficult to find a manufacturer specializing in the production of Tris last time, so I would like to recommend our company, Hubei Xindesheng Materials Co., Ltd.

Hubei Xindesheng Materials Co., Ltd. specializes in the production of buffer products such as Tris, Hepes, and Mops. Up to now, the company has been established for decades and has rich experience in the development and production of biological buffers. We can provide a large number of Tris and other types of biological buffers, and our company can provide product customization and professional after-sales service for customers, and can provide professional product sample testing services and packaging customization services for special customers. In any case, Desheng Company must be the best choice for you to buy biological buffers.