The difference and preparation of HEPES and HEPES-Na CAS NO.7365-45-9

1. Factory production, more favorable prices, and regular products are available in stock;

2. Desheng company is an enterprise producing biological buffer products, providing you with more comprehensive services with a variety of products;

3. Desheng has been in this industry for 16 years. Our product knowledge and production experience are very rich

4. Desheng has been engaged in foreign trade since 2010. We have our own right to export.

HEPES buffer Chinese full name 4-hydroxyethylpiperazineethanesulfonic acid, CAS number: 7365-45-9, pH buffer range 6.8 - 8.2, at 25 ℃, its pKa value is 7.5. HEPES-Na is N-(2-hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid) sodium salt, and its CAS number is 75277-39-3. Both HEPES and HEPES-Na are very important biological buffers, which are widely used in biopharmaceuticals, medical diagnosis and other fields.

HEPES is a zwitterionic buffer that is used in cell culture media and protein research for various types of organisms. It can also be used in biochemical diagnostic kits, DNA/RNA extraction kits and PCR diagnostic kits. It has the advantages of toxic effect and can control a constant pH range for a long time.
The difference between HEPES and HEPES-Na: HEPES-Na is also known as organic HEPES base, and HEPES is a conjugate acid-base relationship. The two are essentially the same, but the pH of the two substances after dissolution is different.
Preparation method of HEPES:
Regarding the preparation of HEPES buffer solution, according to the use, one is pure HEPES + NaOH, and the other is HEPES + salt. The various preparation methods are summarized as follows:
1. Preparation of 500ml 1 M HEPES, pH = 7.0 Stock solution
Dissolve 119.15 g of HEPES in 400 ml of distilled water, add 0.5-1M NaOH aqueous solution to adjust at least the required pH (the effective pH range of HEPES is 6.8-8.2), then dilute to 500 ml with distilled water, and store at 4 degrees Celsius.
2. HEPES Buffer formula with a small amount of salt (500ml)
HEPES 6.5g, NaCl 8.0g, Na2HPO4.7H2O 0.198g, adjust the pH value with 0.5M NaOH aqueous solution, and finally set the volume.
3. Preparation of 2 × HEPES buffered saline solution
Dissolve 1.6g NaCl, 0.074g KCl, 0.027g Na2HPO4.2H2O, 0.2g dextran (glucan or dextran) and 1g HEPES in 90ml of distilled water, adjust to the desired pH value with 0.5M NaOH, and dilute to volume with distilled water to 100ml.
Preparation method of HEPES-Na:
HEPES-Na can be synthesized by 4-hydroxyethylpiperazine and sodium vinylsulfonate, or it can be synthesized by high pressure using isethionic acid, sodium hydroxide and 4-hydroxyethylpiperazine. At present, it meets the requirements of biological buffers. The most extensive preparation method is to react HEPES with NaOH to form HEPES-Na. The specific preparation steps are as follows:
Under nitrogen protection, the solid or solution of HEPES and NaOH with a purity of 90-99% is reacted for 0.2-1 hour, and the reaction temperature is 20-100 ° C. After the reaction is completed, the obtained product is decolorized, filtered, concentrated, and dewatered. , crystallization, washing, drying and other operations to obtain HEPES-Na.

The preparation method has the advantages of simple process, convenient operation, high yield of the obtained HEPES-Na product, and high product purity, which can meet the purity requirements of the biological buffer.
Desheng develops and produces a complete range of biological buffer series products, has in-depth research, selects materials for production and packaging, and insists on providing customers with high-quality raw materials.